| Product Name | Bovine Methionine synthase reductase (MTRR) ELISA Kit |
|---|---|
| Description | Bovine Methionine synthase reductase (MTRR) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine MTRR. No significant cross-reactivity or interference between Bovine MTRR and analogues was observed. Methionine is an essential amino acid required for protein synthesis and one-carbon metabolism. Its synthesis is catalyzed by the enzyme methionine synthase. Methionine synthase eventually becomes inactive due to the oxidation of its cob(I)alamin cofactor. MTRR encoded by this gene regenerates a functional methionine synthase via reductive methylation. It is a member of the ferredoxin-NADP(+) reductase (FNR) family of electron transferases. Patients of the cbl-E complementation group of disorders of folate/cobalamin metabolism are defective in reductive activation of methionine synthase. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. This Bovine Methionine synthase reductase (MTRR) ELISA Kit employs a two-site sandwich ELISA to quantitate MTRR in samples. An antibody specific for MTRR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTRR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTRR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTRR bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | MTRR, MGC129643, MSR, cblE, [methionine synthase]-cobalamin methyltransferase (cob(II)alamin reducing), methionine synthase reductase |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 280869 |
| Storage | 2-8°C |
| Background |
Methionine is an essential amino acid required for protein synthesis and one-carbon metabolism. Its synthesis is catalyzed by the enzyme methionine synthase. Methionine synthase eventually becomes inactive due to the oxidation of its cob(I)alamin cofactor. MTRR encoded by this gene regenerates a functional methionine synthase via reductive methylation. It is a member of the ferredoxin-NADP(+) reductase (FNR) family of electron transferases. Patients of the cbl-E complementation group of disorders of folate/cobalamin metabolism are defective in reductive activation of methionine synthase. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. |
| Supplier | Abbkine |
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