Bovine Microtubule-associated protein RP/EB family member 2 (MAPRE2) ELISA Kit
| Product Name | Bovine Microtubule-associated protein RP/EB family member 2 (MAPRE2) ELISA Kit |
|---|---|
| Description | Bovine Microtubule-associated protein RP/EB family member 2 (MAPRE2) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine MAPRE2. No significant cross-reactivity or interference between Bovine MAPRE2 and analogues was observed. MAPRE2 shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. The function of this protein is unknown; however, its homology suggests involvement in tumorigenesis of colorectal cancers and proliferative control of normal cells. This gene may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR. The deduced 327-amino acid protein has significant homology with EB1 family proteins. Northern blot analysis detected a 2.6-kb transcript in T cells activated by 2 signals (i.e., cell surface antigen(s) and/or cytokine) and also in lymphocyte tumor cell lines. Immunoprecipitation analysis indicated that RP1 associates with full-length but not C terminus-deleted APC. This Bovine Microtubule-associated protein RP/EB family member 2 (MAPRE2) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPRE2 in samples. An antibody specific for MAPRE2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPRE2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPRE2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPRE2 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | MAPRE2, EB1, EB2, RP1, APC-binding protein EB1, T-cell activation protein, EB1 family |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 510159 |
| Storage | 2-8°C |
| Background |
MAPRE2 shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. The function of this protein is unknown; however, its homology suggests involvement in tumorigenesis of colorectal cancers and proliferative control of normal cells. This gene may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR. The deduced 327-amino acid protein has significant homology with EB1 family proteins. Northern blot analysis detected a 2.6-kb transcript in T cells activated by 2 signals (i.e., cell surface antigen(s) and/or cytokine) and also in lymphocyte tumor cell lines. Immunoprecipitation analysis indicated that RP1 associates with full-length but not C terminus-deleted APC. |
| Supplier | Abbkine |
All Research Products are sold for laboratory RESEARCH USE ONLY and ARE NOT TO BE USED FOR HUMAN OR ANIMAL THERAPEUTIC OR DIAGNOSTIC APPLICATIONS. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. ARP American Research Products, Inc. does not submit its products for regulatory review by any government body or other organization, and we do not validate them for clinical, therapeutic or diagnostic use, or for safety and effectiveness. You are solely responsible for making sure that the way you use the products complies with applicable laws, regulations and governmental policies and for obtaining all necessary approvals, intellectual property rights, licenses and permissions that you may need related to your use. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process. ARP American Research Products, Inc. disclaims any and all representations or warranties of any kind whatsoever, express or implied, including without limitation any implied warranties of merchantability or fitness for a particular purpose, of non-infringement, or regarding results obtained through the use of any product, whether arising from a statute or otherwise in law or from a course of performance, dealing or usage of trade.
