| Product Name | Bovine MIF4G domain-containing protein (MIF4GD) ELISA Kit |
|---|---|
| Description | Bovine MIF4G domain-containing protein (MIF4GD) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine MIF4GD. No significant cross-reactivity or interference between Bovine MIF4GD and analogues was observed. MIF4G domain-containing protein (MIF4GD) gene encodes a protein which interacts with the N-terminus of the stem-loop binding protein (SLBP) and the 3' end of histone mRNA. This interaction facilitates the activation of histone mRNA translation. Alternative splicing results in multiple transcript variants encoding different isoforms. This Bovine MIF4G domain-containing protein (MIF4GD) ELISA Kit employs a two-site sandwich ELISA to quantitate MIF4GD in samples. An antibody specific for MIF4GD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMIF4GD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MIF4GD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MIF4GD bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | MIF4GD, AD023, MGC45027, MIFD, SLIP1, SLBP (stem loop binding protein)-interacting protein 1 |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 613305 |
| Storage | 2-8°C |
| Background | MIF4G domain-containing protein (MIF4GD) gene encodes a protein which interacts with the N-terminus of the stem-loop binding protein (SLBP) and the 3' end of histone mRNA. This interaction facilitates the activation of histone mRNA translation. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Supplier | Abbkine |
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