Bovine Mitochondrial import receptor subunit TOM5 homolog (TOMM5) ELISA Kit
| Product Name | Bovine Mitochondrial import receptor subunit TOM5 homolog (TOMM5) ELISA Kit |
|---|---|
| Description | Bovine Mitochondrial import receptor subunit TOM5 homolog (TOMM5) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine TOMM5. No significant cross-reactivity or interference between Bovine TOMM5 and analogues was observed. The fungal preprotein translocase of the mitochondrial outer membrane (TOM complex) comprises import receptors Tom70, Tom20, and Tom22, import channel Tom40, and small Tom proteins Tom5, Tom6, and Tom7, which regulate TOM complex assembly. These components are conserved in mammals; unlike the other components, however, Tom5 and Tom6 remain unidentified in mammals. These small Tom proteins are associated with Tom40 in the TOM complex. Knockdown of Tom7, but not Tom5 and Tom6, strongly compromised stability of the TOM complex. Conversely, knockdown of hTom40 decreased the level of all small Tom proteins. Matrix import of preprotein was affected by double knockdown of any combination of small Tom proteins. These results indicate that human small Tom proteins maintain the structural integrity of the TOM complex. This Bovine Mitochondrial import receptor subunit TOM5 homolog (TOMM5) ELISA Kit employs a two-site sandwich ELISA to quantitate TOMM5 in samples. An antibody specific for TOMM5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTOMM5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOMM5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOMM5 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | TOMM5, RP11-613M10.3, C9orf105, RP11-263I4.1, Tom5, bA613M10.3, mitochondrial outer membrane protein TOM5, translocase of outer mitochondrial membrane 5 homolog |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 615897 |
| Storage | 2-8°C |
| Background | The fungal preprotein translocase of the mitochondrial outer membrane (TOM complex) comprises import receptors Tom70, Tom20, and Tom22, import channel Tom40, and small Tom proteins Tom5, Tom6, and Tom7, which regulate TOM complex assembly. These components are conserved in mammals; unlike the other components, however, Tom5 and Tom6 remain unidentified in mammals. These small Tom proteins are associated with Tom40 in the TOM complex. Knockdown of Tom7, but not Tom5 and Tom6, strongly compromised stability of the TOM complex. Conversely, knockdown of hTom40 decreased the level of all small Tom proteins. Matrix import of preprotein was affected by double knockdown of any combination of small Tom proteins. These results indicate that human small Tom proteins maintain the structural integrity of the TOM complex. |
| Supplier | Abbkine |
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