| Product Name | Bovine Myosin-1 (MYH1) ELISA Kit |
|---|---|
| Description | Bovine Myosin-1 (MYH1) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine MYH1. No significant cross-reactivity or interference between Bovine MYH1 and analogues was observed. Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development. This Bovine Myosin-1 (MYH1) ELISA Kit employs a two-site sandwich ELISA to quantitate MYH1 in samples. An antibody specific for MYH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYH1 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | MYH1, MGC133384, MYHSA1, MYHa, MyHC-2X/D, myosin heavy chainx/d, myosin, heavy polypeptide 1, skeletal muscle, adult |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 281337 |
| Storage | 2-8°C |
| Background | Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. Myosin heavy chains are encoded by a multigene family. In mammals at least 10 different myosin heavy chain (MYH) isoforms have been described from striated, smooth, and nonmuscle cells. These isoforms show expression that is spatially and temporally regulated during development. |
| Supplier | Abbkine |
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