| Product Name | Bovine Myosin regulatory light chain 12B (MYL12B) ELISA Kit |
|---|---|
| Description | Bovine Myosin regulatory light chain 12B (MYL12B) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine MYL12B. No significant cross-reactivity or interference between Bovine MYL12B and analogues was observed. The activity of nonmuscle myosin II is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments. This Bovine Myosin regulatory light chain 12B (MYL12B) ELISA Kit employs a two-site sandwich ELISA to quantitate MYL12B in samples. An antibody specific for MYL12B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYL12B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYL12B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYL12B bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | MYL12B, MLC-B, MRLC2, OTTHUMP00000165808, myosin regulatory light chain 2, myosin regulatory light chain MRCL2, myosin regulatory light chain MRLC2 |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 614055 |
| Storage | 2-8°C |
| Background | The activity of nonmuscle myosin II is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments |
| Supplier | Abbkine |
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