| Product Name | Bovine Myosin regulatory light polypeptide 9 (MYL9) ELISA Kit |
|---|---|
| Description | Bovine Myosin regulatory light polypeptide 9 (MYL9) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine MYL9. No significant cross-reactivity or interference between Bovine MYL9 and analogues was observed. Myosin, a structural component of muscle, consists of two heavy chains and four light chains. Myosin regulatory light polypeptide 9 (MYL9) is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. MYL9 binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for MYL9. This Bovine Myosin regulatory light polypeptide 9 (MYL9) ELISA Kit employs a two-site sandwich ELISA to quantitate MYL9 in samples. An antibody specific for MYL9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYL9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYL9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYL9 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | MYL9, LC20, MGC3505, MLC2, MRLC1, MYRL2, myosin RLC, myosin regulatory light chain 1, myosin regulatory light chain 2, smooth muscle isoform, myosin regulatory light chain 9, myosin, light polypeptide 9, regu |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 531505 |
| Storage | 2-8°C |
| Background | Myosin, a structural component of muscle, consists of two heavy chains and four light chains. Myosin regulatory light polypeptide 9 (MYL9) is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. MYL9 binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for MYL9. |
| Supplier | Abbkine |
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