Bovine Peptide chain release factor 1, mitochondrial (MTRF1) ELISA Kit
| Product Name | Bovine Peptide chain release factor 1, mitochondrial (MTRF1) ELISA Kit |
|---|---|
| Description | Bovine Peptide chain release factor 1, mitochondrial (MTRF1) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine MTRF1. No significant cross-reactivity or interference between Bovine MTRF1 and analogues was observed. Peptide chain release factor 1, mitochondrial (MTRF1) was determined by in silico methods to be a mitochondrial protein with similarity to the peptide chain release factors (RFs) discovered in bacteria and yeast. The peptide chain release factors direct the termination of translation in response to the peptide chain termination codons. Initially thought to have a role in the termination of mitochondria protein synthesis, a recent publication found no mitochondrial translation release functionality. Multiple alternatively spliced transcript variants have been suggested by mRNA and EST data; however, their full-length natures are not clear. This Bovine Peptide chain release factor 1, mitochondrial (MTRF1) ELISA Kit employs a two-site sandwich ELISA to quantitate MTRF1 in samples. An antibody specific for MTRF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTRF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTRF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTRF1 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | MTRF1, RP11-74J13.3, MGC47721, MRF1, MTTRF1, RF1, OTTHUMP00000018316, mitochontrial peptide chain release factor 1 |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 504754 |
| Storage | 2-8°C |
| Background | Peptide chain release factor 1, mitochondrial (MTRF1) was determined by in silico methods to be a mitochondrial protein with similarity to the peptide chain release factors (RFs) discovered in bacteria and yeast. The peptide chain release factors direct the termination of translation in response to the peptide chain termination codons. Initially thought to have a role in the termination of mitochondria protein synthesis, a recent publication found no mitochondrial translation release functionality. Multiple alternatively spliced transcript variants have been suggested by mRNA and EST data; however, their full-length natures are not clear. |
| Supplier | Abbkine |
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