| Product Name | Bovine Peroxisomal membrane protein 2 (PXMP2) ELISA Kit |
|---|---|
| Description | Bovine Peroxisomal membrane protein 2 (PXMP2) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine PXMP2. No significant cross-reactivity or interference between Bovine PXMP2 and analogues was observed. PMP22 is a major component of peroxisomal membranes in mammals.It seems to be involved in pore forming activity and may contribute to the unspecific permeability of the organelle membrane. PMP22 is synthesized on free cytosolic ribosomes and then directed to the peroxisome membrane by specific targeting information. Previous studies in rats revealed that PMP22 contains one distinct peroxisomal membrane targeting signal in the amino-terminal cytoplasmic tail. Amino acid sequence alignment of rat and human protein revealed 77% identity including a high conservation of several protein motifs.Human as well as rat PMP22 contains two distinct and nonoverlapping peroxisomal membrane targeting signals, one in the amino-terminal and the other in the carboxyl-terminal protein region. This Bovine Peroxisomal membrane protein 2 (PXMP2) ELISA Kit employs a two-site sandwich ELISA to quantitate PXMP2 in samples. An antibody specific for PXMP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPXMP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PXMP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PXMP2 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | PXMP2, FLJ54922, PMP22, |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 508609 |
| Storage | 2-8°C |
| Background | PMP22 is a major component of peroxisomal membranes in mammals.It seems to be involved in pore forming activity and may contribute to the unspecific permeability of the organelle membrane. PMP22 is synthesized on free cytosolic ribosomes and then directed to the peroxisome membrane by specific targeting information. Previous studies in rats revealed that PMP22 contains one distinct peroxisomal membrane targeting signal in the amino-terminal cytoplasmic tail. Amino acid sequence alignment of rat and human protein revealed 77% identity including a high conservation of several protein motifs.Human as well as rat PMP22 contains two distinct and nonoverlapping peroxisomal membrane targeting signals, one in the amino-terminal and the other in the carboxyl-terminal protein region. |
| Supplier | Abbkine |
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