| Product Name | Bovine Platelet Factor 4 (PF-4) ELISA Kit |
|---|---|
| Description | Bovine Platelet Factor 4 (PF-4) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine PF-4. No significant cross-reactivity or interference between Bovine PF-4 and analogues was observed. Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family. This chemokine is released from alpha-granules of activated platelets during platelet aggregation, and promotes blood coagulation by moderating the effects of heparin-like molecules. Due to these roles, it is predicted to play a role in wound repair and inflammation. It is usually found in a complex with proteoglycan.PF4 is chemotactic for neutrophils, fibroblasts and monocytes, and interacts with a splice variant of the chemokine receptor CXCR3, known as CXCR3B. PF4 is the antigen in heparin-induced thrombocytopenia, an idiosyncratic autoimmune reaction to the administration of the anticoagulant heparin. PF4 autoantibodies have also been found in patients with thrombosis and features resembling HIT but no prior administration of heparin. This Bovine Platelet Factor 4 (PF-4) ELISA Kit employs a two-site sandwich ELISA to quantitate PF-4 in samples. An antibody specific for PF-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPF-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PF-4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PF-4 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | PF-4, CXCL4, MGC138298, SCYB4, chemokine (C-X-C motif) ligand 4, platelet factor 4 (chemokine (C-X-C motif) ligand 4) |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Storage | 2-8°C |
| Background | Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family. This chemokine is released from alpha-granules of activated platelets during platelet aggregation, and promotes blood coagulation by moderating the effects of heparin-like molecules. Due to these roles, it is predicted to play a role in wound repair and inflammation. It is usually found in a complex with proteoglycan.PF4 is chemotactic for neutrophils, fibroblasts and monocytes, and interacts with a splice variant of the chemokine receptor CXCR3, known as CXCR3B. PF4 is the antigen in heparin-induced thrombocytopenia, an idiosyncratic autoimmune reaction to the administration of the anticoagulant heparin. PF4 autoantibodies have also been found in patients with thrombosis and features resembling HIT but no prior administration of heparin. |
| Supplier | Abbkine |
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