| Product Name | Bovine Prolactin-releasing peptide (PRLH) ELISA Kit |
|---|---|
| Description | Bovine Prolactin-releasing peptide (PRLH) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine PRLH. No significant cross-reactivity or interference between Bovine PRLH and analogues was observed. PRH mRNA was expressed in all 5 normal pituitary glands examined. In contrast, PRH mRNA was detected in only 5 of 11 human prolactinomas. All 5 prolactinomas expressing PRH were responsive to dopamine agonist treatment, whereas PRH-negative prolactinomas were non- or partially responsive. PRH mRNA was also detected in 6 of 13 GH-secreting tumors and in 5 of 10 clinically nonfunctioning tumors investigated. PRH receptor mRNA was found in all the normal and neoplastic human pituitary samples studied. The authors concluded that the production of PRH and its receptor by normal and neoplastic pituitary tissue raises the question of whether it may regulate prolactin production in an autocrine/paracrine manner in pituitary tissue. This Bovine Prolactin-releasing peptide (PRLH) ELISA Kit employs a two-site sandwich ELISA to quantitate PRLH in samples. An antibody specific for PRLH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRLH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRLH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRLH bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | PRLH, PRH, PRRP, preproprolactin-releasing peptide |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 286856 |
| Storage | 2-8°C |
| Background |
PRH mRNA was expressed in all 5 normal pituitary glands examined. In contrast, PRH mRNA was detected in only 5 of 11 human prolactinomas. All 5 prolactinomas expressing PRH were responsive to dopamine agonist treatment, whereas PRH-negative prolactinomas were non- or partially responsive. PRH mRNA was also detected in 6 of 13 GH-secreting tumors and in 5 of 10 clinically nonfunctioning tumors investigated. PRH receptor mRNA was found in all the normal and neoplastic human pituitary samples studied. The authors concluded that the production of PRH and its receptor by normal and neoplastic pituitary tissue raises the question of whether it may regulate prolactin production in an autocrine/paracrine manner in pituitary tissue. |
| Supplier | Abbkine |
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