| Product Name | Bovine Protein Mdm4 (MDM4) ELISA Kit |
|---|---|
| Description | Bovine Protein Mdm4 (MDM4) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine MDM4. No significant cross-reactivity or interference between Bovine MDM4 and analogues was observed. Protein Mdm4 (MDM4) gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. This Bovine Protein Mdm4 (MDM4) ELISA Kit employs a two-site sandwich ELISA to quantitate MDM4 in samples. An antibody specific for MDM4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMDM4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MDM4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MDM4 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | MDM4, RP11-430C7.1, DKFZp781B1423, HDMX, MDMX, MGC132766, MRP1, MDM4-related protein 1, Mdm4, transformed 3T3 cell double minute 4, p53 binding protein, double minute 4, human homolog of, p53-binding protei |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 514225 |
| Storage | 2-8°C |
| Background | Protein Mdm4 (MDM4) gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. |
| Supplier | Abbkine |
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