| Product Name | Bovine Protein spinster homolog 1 (SPNS1) ELISA Kit |
|---|---|
| Description | Bovine Protein spinster homolog 1 (SPNS1) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine SPNS1. No significant cross-reactivity or interference between Bovine SPNS1 and analogues was observed. SPIN1 played a critical role in necrotic cell death in cultured human cells. SPIN1 bound the antiapoptotic protein BCL2 and the apoptosis regulator BCLXL and induced cell death via a pathway that was independent of mitochondrial cytochrome c (CYCS) release and caspase activation. Mutation analysis showed that SPIN1 bound BCLXL via its BH3 domain. Necrosis inhibitors, but not caspase inhibitors, blocked SPIN1-induced cell death. SPIN1-induced cell death increased autophagic vacuole formation and the mature form of lysosomal cathepsin D (CTSD), suggesting SPIN1 induces necrotic cell death via autophagy.SPIN1 expression was also detected in HeLa and HEK293 cell lines. SPIN1 localized diffusely throughout the cytoplasm and concentrated in a punctate pattern that colocalized with a mitochondrial marker. This Bovine Protein spinster homolog 1 (SPNS1) ELISA Kit employs a two-site sandwich ELISA to quantitate SPNS1 in samples. An antibody specific for SPNS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPNS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPNS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPNS1 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | SPNS1, FLJ38358, HSpin1, LAT, PP2030, SPIN1, SPINL, nrs, spinster homolog 1, spinster-like |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 505997 |
| Storage | 2-8°C |
| Background | SPIN1 played a critical role in necrotic cell death in cultured human cells. SPIN1 bound the antiapoptotic protein BCL2 and the apoptosis regulator BCLXL and induced cell death via a pathway that was independent of mitochondrial cytochrome c (CYCS) release and caspase activation. Mutation analysis showed that SPIN1 bound BCLXL via its BH3 domain. Necrosis inhibitors, but not caspase inhibitors, blocked SPIN1-induced cell death. SPIN1-induced cell death increased autophagic vacuole formation and the mature form of lysosomal cathepsin D (CTSD), suggesting SPIN1 induces necrotic cell death via autophagy.SPIN1 expression was also detected in HeLa and HEK293 cell lines. SPIN1 localized diffusely throughout the cytoplasm and concentrated in a punctate pattern that colocalized with a mitochondrial marker. |
| Supplier | Abbkine |
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