Bovine Pyrroline-5-carboxylate reductase 1, mitochondrial (PYCR1) ELISA Kit
| Product Name | Bovine Pyrroline-5-carboxylate reductase 1, mitochondrial (PYCR1) ELISA Kit |
|---|---|
| Description | Bovine Pyrroline-5-carboxylate reductase 1, mitochondrial (PYCR1) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine PYCR1. No significant cross-reactivity or interference between Bovine PYCR1 and analogues was observed. PYCR1 catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternate splicing results in two transcript variants encoding different isoforms.The 1,810-bp cDNA hybridized to a 1.85-kb mRNA in samples from human cell lines and predicated a 319-amino acid, 33.4-kD protein. PYCR1 protein colocalized with various mitochondrial markers, including delta-1-pyrroline-5-carboxylate synthetase (P5CS), which also belongs to the proline metabolic pathway. Knockdown of the orthologous genes in Xenopus and zebrafish led to epidermal hypoplasia and blistering that was accompanied by a massive increase in apoptosis. This Bovine Pyrroline-5-carboxylate reductase 1, mitochondrial (PYCR1) ELISA Kit employs a two-site sandwich ELISA to quantitate PYCR1 in samples. An antibody specific for PYCR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPYCR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PYCR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYCR1 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | PYCR1, ARCL2B, P5C, P5CR, PIG45, PP222, PRO3, PYCR, P5C reductase, proliferation-inducing protein 45 |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 539606 |
| Storage | 2-8°C |
| Background |
PYCR1 catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternate splicing results in two transcript variants encoding different isoforms.The 1,810-bp cDNA hybridized to a 1.85-kb mRNA in samples from human cell lines and predicated a 319-amino acid, 33.4-kD protein. PYCR1 protein colocalized with various mitochondrial markers, including delta-1-pyrroline-5-carboxylate synthetase (P5CS), which also belongs to the proline metabolic pathway. Knockdown of the orthologous genes in Xenopus and zebrafish led to epidermal hypoplasia and blistering that was accompanied by a massive increase in apoptosis. |
| Supplier | Abbkine |
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