| Product Name | Bovine Retinol-binding protein 4 (RBP4) ELISA Kit |
|---|---|
| Description | Bovine Retinol-binding protein 4 (RBP4) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine RBP4. No significant cross-reactivity or interference between Bovine RBP4 and analogues was observed. Retinol binding protein (RBP) 4 is the only specific transport protein for vitamin A in the circulation whose function is to deliver vitamin to target tissues. In obesity and type 2 diabetes, expression of Glut4 is significantly impaired in adipocytes. Glucose transport via Glut4 is the rate-limiting step for glucose use by muscle and adipose tissue. Yang et al. noted that adipocytespecific deletion of Gluts led to notable elevation of RBP4 causing systemic insulin resistance, and that reduction of RBP4 improved insulin resistance. This identified a novel role of RBP4 in regulating insulin action and RBP4 is recorded as an adipocytederived hormone. Thus, measurement of serum or plasma RBP4 is a useful means for understanding of metabolic disorders. This Bovine Retinol-binding protein 4 (RBP4) ELISA Kit employs a two-site sandwich ELISA to quantitate RBP4 in samples. An antibody specific for RBP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRBP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RBP4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RBP4 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | RBP4, OTTHUMP00000020116, retinol-binding protein 4, interstitial, retinol-binding protein 4, plasma |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Storage | 2-8°C |
| Background |
Retinol binding protein (RBP) 4 is the only specific transport protein for vitamin A in the circulation whose function is to deliver vitamin to target tissues. In obesity and type 2 diabetes, expression of Glut4 is significantly impaired in adipocytes. Glucose transport via Glut4 is the rate-limiting step for glucose use by muscle and adipose tissue. Yang et al. noted that adipocytespecific deletion of Gluts led to notable elevation of RBP4 causing systemic insulin resistance, and that reduction of RBP4 improved insulin resistance. This identified a novel role of RBP4 in regulating insulin action and RBP4 is recorded as an adipocytederived hormone. Thus, measurement of serum or plasma RBP4 is a useful means for understanding of metabolic disorders. |
| Supplier | Abbkine |
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