Bovine Serine/threonine-protein kinase greatwall (MASTL) ELISA Kit
| Product Name | Bovine Serine/threonine-protein kinase greatwall (MASTL) ELISA Kit |
|---|---|
| Description | Bovine Serine/threonine-protein kinase greatwall (MASTL) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine MASTL. No significant cross-reactivity or interference between Bovine MASTL and analogues was observed. Gandhi et al. (2003) identified MASTL, which they called FLJ14813, within the region of chromosome 10p where Drachman et al. (2000) had mapped autosomal dominant thrombocytopenia, also known as thrombocytopenia-2 (THC2), by linkage analysis. The protein product of the MASTL gene is a putative kinase that contains 2 highly conserved kinase domains. Gandhi et al. (2003) noted that a similar gene, 'greatwall,' had been described in Drosophila. EMS-induced mutations in the greatwall gene cause a metaphase-arrest phenotype and problems in chromosome condensation.By extensive sequencing of multiple genes located in the critical segment on 10p, Gandhi et al. (2003) demonstrated a missense mutation in the MASTL gene . This Bovine Serine/threonine-protein kinase greatwall (MASTL) ELISA Kit employs a two-site sandwich ELISA to quantitate MASTL in samples. An antibody specific for MASTL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMASTL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MASTL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MASTL bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | MASTL, FLJ14813, RP11-85G18.2, THC2, |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Storage | 2-8°C |
| Background | Gandhi et al. (2003) identified MASTL, which they called FLJ14813, within the region of chromosome 10p where Drachman et al. (2000) had mapped autosomal dominant thrombocytopenia, also known as thrombocytopenia-2 (THC2), by linkage analysis. The protein product of the MASTL gene is a putative kinase that contains 2 highly conserved kinase domains. Gandhi et al. (2003) noted that a similar gene, 'greatwall,' had been described in Drosophila. EMS-induced mutations in the greatwall gene cause a metaphase-arrest phenotype and problems in chromosome condensation.By extensive sequencing of multiple genes located in the critical segment on 10p, Gandhi et al. (2003) demonstrated a missense mutation in the MASTL gene . |
| Supplier | Abbkine |
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