| Product Name | Bovine Synaptojanin-2-binding protein (SYNJ2BP) ELISA Kit |
|---|---|
| Description | Bovine Synaptojanin-2-binding protein (SYNJ2BP) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine SYNJ2BP. No significant cross-reactivity or interference between Bovine SYNJ2BP and analogues was observed. The 3-prime region of the transcript is AU rich and contains multiple mRNA instability repeats. The deduced 206-amino acid protein contains an N-terminal amphipathic helix, followed by a PDZ domain and a C-terminal transmembrane domain. Omp25 mRNA was widely expressed in rat tissues. Omp25 localized to the mitochondrial outer membrane via its C-terminal transmembrane region, with the PDZ domain facing the cytoplasm. Overexpression of Omp25 resulted in perinuclear clustering of mitochondria in transfected cells, and this effect was mimicked by enforced expression of Synj2a on the mitochondrial outer membrane but not by a Synj2a mutant lacking the inositol-5 phosphatase domain. This Bovine Synaptojanin-2-binding protein (SYNJ2BP) ELISA Kit employs a two-site sandwich ELISA to quantitate SYNJ2BP in samples. An antibody specific for SYNJ2BP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYNJ2BP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SYNJ2BP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYNJ2BP bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | SYNJ2BP, ARIP2, FLJ11271, FLJ41973, OMP25, activin receptor interacting protein 5 |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 525107 |
| Storage | 2-8°C |
| Background |
The 3-prime region of the transcript is AU rich and contains multiple mRNA instability repeats. The deduced 206-amino acid protein contains an N-terminal amphipathic helix, followed by a PDZ domain and a C-terminal transmembrane domain. Omp25 mRNA was widely expressed in rat tissues. Omp25 localized to the mitochondrial outer membrane via its C-terminal transmembrane region, with the PDZ domain facing the cytoplasm. Overexpression of Omp25 resulted in perinuclear clustering of mitochondria in transfected cells, and this effect was mimicked by enforced expression of Synj2a on the mitochondrial outer membrane but not by a Synj2a mutant lacking the inositol-5 phosphatase domain. |
| Supplier | Abbkine |
All Research Products are sold for laboratory RESEARCH USE ONLY and ARE NOT TO BE USED FOR HUMAN OR ANIMAL THERAPEUTIC OR DIAGNOSTIC APPLICATIONS. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. ARP American Research Products, Inc. does not submit its products for regulatory review by any government body or other organization, and we do not validate them for clinical, therapeutic or diagnostic use, or for safety and effectiveness. You are solely responsible for making sure that the way you use the products complies with applicable laws, regulations and governmental policies and for obtaining all necessary approvals, intellectual property rights, licenses and permissions that you may need related to your use. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process. ARP American Research Products, Inc. disclaims any and all representations or warranties of any kind whatsoever, express or implied, including without limitation any implied warranties of merchantability or fitness for a particular purpose, of non-infringement, or regarding results obtained through the use of any product, whether arising from a statute or otherwise in law or from a course of performance, dealing or usage of trade.
