Bovine Thioredoxin-related transmembrane protein 2 (TMX2) ELISA Kit

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KTE10030
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Product Name Bovine Thioredoxin-related transmembrane protein 2 (TMX2) ELISA Kit
Description Bovine Thioredoxin-related transmembrane protein 2 (TMX2) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine TMX2. No significant cross-reactivity or interference between Bovine TMX2 and analogues was observed. Thioredoxin plays an important role in various cellular processes through redox regulation. The molecular cloning and characterization of one member of the thioredoxin superfamily, designated as TMX2. The TMX2 cDNA consists of 1644 nucleotides and contains an open reading frame encoding a protein of 372 amino acids with a predicted molecular mass of 42.5 kDa and an isoelectric point of 8.94. The TMX2 protein may possess an N-terminal signal peptide, a potential transmembrane domain, an Myb DNA-binding domain repeat signature, a thioredoxin consensus pattern, an endoplasmic reticulum (ER) membrane retention signal (KKXX-like motif), and a dileucine motif in the tail. Northern blot analysis shows it is widely expressed in human tissues. This Bovine Thioredoxin-related transmembrane protein 2 (TMX2) ELISA Kit employs a two-site sandwich ELISA to quantitate TMX2 in samples. An antibody specific for TMX2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMX2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMX2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMX2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Synonyms TMX2, CGI-31, DKFZp781O2021, MGC111151, PDIA12, PIG26, TXNDC14, growth-inhibiting gene 11, proliferation-inducing gene 26 protein, protein disulfide isomerase family A, member 12, thioredoxin domain containi
Method Sandwich ELISA
Detection Range Please inquire
Sensitivity Please inquire
Reactivity Bovine
Sample Types Cell culture supernatants, Serum, Plasma, Other biological fluids
Gene Id 509244
Storage 2-8°C
Background Thioredoxin plays an important role in various cellular processes through redox regulation. The molecular cloning and characterization of one member of the thioredoxin superfamily, designated as TMX2.
The TMX2 cDNA consists of 1644 nucleotides and contains an open reading frame encoding a protein of 372 amino acids with a predicted molecular mass of 42.5 kDa and an isoelectric point of 8.94. The TMX2 protein may possess an N-terminal signal peptide, a potential transmembrane domain, an Myb DNA-binding domain repeat signature, a thioredoxin consensus pattern, an endoplasmic reticulum (ER) membrane retention signal (KKXX-like motif), and a dileucine motif in the tail. Northern blot analysis shows it is widely expressed in human tissues.
Supplier Abbkine

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