| Product Name | Cow Transmembrane protein 66 (TMEM66) ELISA Kit |
|---|---|
| Description | Transmembrane protein 66 play important roles in cell apoptosis and tumorigenesis. In order to investigate the function of TMEM66 gene, the transgenic mouse model containing the mutant TMEM66 gene was constructed in this study. The transgenic mice were generated by u-sing microinjection method and 312 injected zygotes were implanted into 13 foster mothers. The positive transgenic mice were confirmed by PCR and Southern blotting. Also, the stability of the transgene was confirmed by passage and the way of integration of the transgene was studied using inverse PCR method. In-verse PCR results indicated that the transgenic DNA fragment was integrated into the host ge-nome in a serial multi-copy manner. Therefore, the transgenic mice model containing the mutant TMEM66 gene was constructed successfully. This assay has high sensitivity and excellent specificity for detection of Bovine TMEM66. No significant cross-reactivity or interference between Bovine TMEM66 and analogues was observed. |
| Synonyms | FLJ22274, FOAP-7, HSPC035, MGC8721, XTP3, |
| Method | Sandwich ELISA |
| Detection Range | Request Information |
| Sensitivity | Request Information |
| Reactivity | Bovine |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background | Transmembrane protein 66 play important roles in cell apoptosis and tumorigenesis. In order to investigate the function of TMEM66 gene, the transgenic mouse model containing the mutant TMEM66 gene was constructed in this study. The transgenic mice were generated by u-sing microinjection method and 312 injected zygotes were implanted into 13 foster mothers. The positive transgenic mice were confirmed by PCR and Southern blotting. Also, the stability of the transgene was confirmed by passage and the way of integration of the transgene was studied using inverse PCR method. In-verse PCR results indicated that the transgenic DNA fragment was integrated into the host ge-nome in a serial multi-copy manner. Therefore, the transgenic mice model containing the mutant TMEM66 gene was constructed successfully. |
| Supplier | Abebio |
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