| Product Name | Bovine Ubiquitin B (UBB) ELISA Kit |
|---|---|
| Description | Bovine Ubiquitin B (UBB) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine UBB. No significant cross-reactivity or interference between Bovine UBB and analogues was observed. Ubiquitin is a protein encoded by the UBB gene.one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. This Bovine Ubiquitin B (UBB) ELISA Kit employs a two-site sandwich ELISA to quantitate UBB in samples. An antibody specific for UBB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBB bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | UBB, FLJ25987, MGC8385, OTTHUMP00000064960, OTTHUMP00000064961, polyubiquitin B |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 281370 |
| Storage | 2-8°C |
| Background | Ubiquitin is a protein encoded by the UBB gene.one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome. |
| Supplier | Abbkine |
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