COX2 ELISA kit (Human)

Category: Assay Kits
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SED284Hu
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Product Name COX2 ELISA kit (Human)
Description This assay has high sensitivity and excellent specificity for detection of Cytochrome C Oxidase Subunit II (COX2). No significant cross-reactivity or interference between Cytochrome C Oxidase Subunit II (COX2) and analogues was observed.
Synonyms MTCO2; MT-CO2; COII; COXII; Mitochondrially Encoded Cytochrome C Oxidase II; Cytochrome c oxidase polypeptide II
Method Sandwich
Detection Range 0.156-10ng/mL
Sensitivity 0.054ng/mL
Target COX2
Specificity This assay has high sensitivity and excellent specificity for detection of Cytochrome C Oxidase Subunit II (COX2). No significant cross-reactivity or interference between Cytochrome C Oxidase Subunit II (COX2) and analogues was observed.
Reactivity Human
Sample Types Tissue Homogenates, Cell Lysates And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately.
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References The effects of particulate matter on inflammation of respiratory system: Differences betweenmale and female.;Polar extract of protects cartilage homeostasis: possible mechanism of action;Barrier Strengthening and Anti-inflammatory Effect of Cucurbit Fruits on Intestinal Epithelial Cells In-vitro;Chandrasekaran Chinampudur Velusami, Edwin Jothie Richard &;1, 25-(OH) 2D3 protects Schwann cells against advanced glycation end products-induced apoptosis through PKA-NF-κB pathway;Regulation and Mechanism of miR-518d through the PPARα-Mediated NF-κB Pathway in the Development of Gestational Diabetes Mellitus;Association between Serum Epidermal Growth Factor Receptor and Cyclooxygenase-2 Levels in Patients with Non-small Cell Carcinoma of Lung;Targeting NF-¦ B mediated cell signaling pathway and inflammatory mediators by 1, 2-diazole in A549 cells in vitro;
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