| Product Name | COX2 ELISA kit (Rat) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Cytochrome C Oxidase Subunit II (COX2). No significant cross-reactivity or interference between Cytochrome C Oxidase Subunit II (COX2) and analogues was observed. |
| Synonyms | MTCO2; MT-CO2; COII; COXII; Mitochondrially Encoded Cytochrome C Oxidase II; Cytochrome c oxidase polypeptide II |
| Method | Sandwich |
| Detection Range | 0.312-20ng/mL |
| Sensitivity | 0.124ng/mL |
| Target | COX2 |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Cytochrome C Oxidase Subunit II (COX2). No significant cross-reactivity or interference between Cytochrome C Oxidase Subunit II (COX2) and analogues was observed. |
| Reactivity | Rat |
| Sample Types | Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Uniprot | P00406 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Docking Studies and Biological Evaluation of a Potential β-Secretase Inhibitor of 3-Hydroxyhericenone F from Hericium erinaceus.;Soy isoflavone genistein attenuates lipopolysaccharide-induced cognitive impairments in the rat via exerting anti-oxidative and anti-inflammatory effects;Effect of single and repeated heat stress on chemical signals of heat shock response cascade in the rat's heart.;Quercetin‐3‐methyl ether inhibits esophageal carcinogenesis by targeting the AKT/mTOR/p70S6K and MAPK pathways;Celecoxib prevents cognitive impairment and neuroinflammation in soluble Amyloid β-treated rats;Berberine ameliorates lipopolysaccharide-induced learning and memory deficit in the rat: insights into underlying molecular mechanisms;Trigonelline protects hippocampus against intracerebral Aβ (1–40) as a model of Alzheimer's disease in the rat: insights into underlying mechanisms;Polydatin ameliorates chemotherapy-induced cognitive impairment (chemobrain) by inhibiting oxidative stress, inflammatory response, and apoptosis in rats; |
| Supplier | Cloud Clone |
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