| Product Name | CRF ELISA kit (Rat) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Corticotropin Releasing Hormone (CRH). No significant cross-reactivity or interference between Corticotropin Releasing Hormone (CRH) and analogues was observed. |
| Synonyms | CRH; Corticotropin Releasing Hormone; Corticoliberin |
| Method | Competitive |
| Detection Range | 12.35-1,000pg/mL |
| Sensitivity | 5.53pg/mL |
| Target | CRF |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Corticotropin Releasing Factor (CRF). No significant cross-reactivity or interference between Corticotropin Releasing Factor (CRF) and analogues was observed. |
| Reactivity | Rat |
| Sample Types | Serum, Plasma And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix.Incubate 1 hour at 37℃; 3.Aspirate and wash 3 times; 4.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 5.Aspirate and wash 5 times; 6.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 7.Add 50uL Stop Solution.Read at 450 nm immediately. |
| Uniprot | P01143 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male rats;Differential stress response in rats subjected to chronic mild stress is accompanied by changes in CRH-family gene expression at the pituitary level;Acute Stress-Induced Changes in Follicular Dermal Papilla Cells and Mobilization of Mast Cells:Implications for Hair Growth.;Heat stress-induced neuroinflammation and aberration in monoamine levels in hypothalamus are associated with temperature dysregulation;Effects of n-3 PUFA enriched and n-3 PUFA deficient diets in naïve and Aβ-treated female rats;Perfluorooctane sulfonate (PFOS) can alter the hypothalamic-pituitary-adrenal (HPA) axis activity by modifying CRF1 and glucocorticoid receptors; |
| Supplier | Cloud Clone |
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