CRP ELISA kit (Rat)

Category: Assay Kits
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HEA821Ra
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Product Name CRP ELISA kit (Rat)
Description This assay has high sensitivity and excellent specificity for detection of High Sensitive C Reactive Protein (CRP). No significant cross-reactivity or interference between High Sensitive C Reactive Protein (CRP) and analogues was observed.
Synonyms C-RP; PTX1; Pentraxin-Related; Pentraxin 1
Method Sandwich
Detection Range 15.6-1,000pg/mL
Sensitivity 7.4pg/mL
Target CRP
Specificity This assay has high sensitivity and excellent specificity for detection of High Sensitive C Reactive Protein (CRP). No significant cross-reactivity or interference between High Sensitive C Reactive Protein (CRP) and analogues was observed.
Reactivity Rat
Sample Types Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately.
Uniprot P48199
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References Perirenal fat associated with microalbuminuria in obese rats;As If Biomarker Discovery Isn't Hard Enough: The Consequences of Poorly Characterized Reagents; Induction of Haemeoxygenase-1 Improves FFA-Induced Endothelial Dysfunction in Rat Aorta;Induction of Haemeoxygenase-1 Directly Improves Endothelial Function in Isolated Aortas from Obese Rats through the Ampk-Pi3k/Akt-Enos Pathway;Renal protective effects of induction of haem oxygenase-1 combined with increased adiponectin on the glomerular vascular endothelial growth factor–nitric oxide axis in obese rats;Protective Effects of Statin and Angiotensin Receptor Blocker in a Rat Model of Doxorubicin-and Trastuzumab-Induced Cardiomyopathy;Perfluorooctane sulfonate exerts inflammatory bowel disease-like intestinal injury in rats;
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