| Product Name | CSF1, 33-255aa, Human, Baculovirus (Bioactivity Validated) |
|---|---|
| Description | CSF1, also known as macrophage colony-stimulating factor 1, is a hematopoietic growth factor that is the primary regulator of the survival, proliferation, differentiation and function of mononuclear phagocytes. It is a four alpha-helical bundle cytokine and its active form is found extracellularly as a disulfide linked homodimer. This protein plays important roles in innate immunity, cancer and inflammatory diseases, including systemic lupus erythematosus, arthritis, atherosclerosis and obesity. In several conditions, activation of macrophages involves a CSF1 autocrine loop. In addition, secreted and cell-surface isoforms of CSF1 can have differential effects in inflammation and immunity. Recombinant human CSF1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Synonyms | Macrophage colony-stimulating factor 1, CSF1, M-CSF, MCSF, Lanimostim |
| Host | Virus |
| Molecular Weight | 15.9 kDa (145aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK ACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQD VVTKPDCNCL YPKAIPSSDP ASVSPHQPLA PSMAPVAGLT WEDSEGTEGS SLLPGEQPLH TVDPGSAKQR PPRLEHHHHH H |
| Tag | His-tag |
| Form | Liquid, in 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 2mM DTT, 10% glycerol |
| Concentration | 0.5 mg/ml (determined by Bradford assay) |
| Purity | > 95 % by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Chitu V., et al. (2006) Curr Opin Immunol. 18(1):39-48. Eda H., et al. (2010) Cytokine. 52(3):215-220. Tian F., et al. (2013) Oncol. Res. 20(8):341-350. |
| Background | CSF1, also known as macrophage colony-stimulating factor 1, is a hematopoietic growth factor that is the primary regulator of the survival, proliferation, differentiation and function of mononuclear phagocytes. It is a four alpha-helical bundle cytokine and its active form is found extracellularly as a disulfide linked homodimer. This protein plays important roles in innate immunity, cancer and inflammatory diseases, including systemic lupus erythematosus, arthritis, atherosclerosis and obesity. In several conditions, activation of macrophages involves a CSF1 autocrine loop. In addition, secreted and cell-surface isoforms of CSF1 can have differential effects in inflammation and immunity. Recombinant human CSF1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Supplier | ARP |
All Research Products are sold for laboratory RESEARCH USE ONLY and ARE NOT TO BE USED FOR HUMAN OR ANIMAL THERAPEUTIC OR DIAGNOSTIC APPLICATIONS. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. ARP American Research Products, Inc. does not submit its products for regulatory review by any government body or other organization, and we do not validate them for clinical, therapeutic or diagnostic use, or for safety and effectiveness. You are solely responsible for making sure that the way you use the products complies with applicable laws, regulations and governmental policies and for obtaining all necessary approvals, intellectual property rights, licenses and permissions that you may need related to your use. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process. ARP American Research Products, Inc. disclaims any and all representations or warranties of any kind whatsoever, express or implied, including without limitation any implied warranties of merchantability or fitness for a particular purpose, of non-infringement, or regarding results obtained through the use of any product, whether arising from a statute or otherwise in law or from a course of performance, dealing or usage of trade.
