CSN2 ELISA kit (Bovine)

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SEJ332Bo
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Product Name CSN2 ELISA kit (Bovine)
Description This assay has high sensitivity and excellent specificity for detection of Casein Beta (CSN2). No significant cross-reactivity or interference between Casein Beta (CSN2) and analogues was observed.
Synonyms CASb
Method Sandwich
Detection Range 4.7-300ng/mL
Sensitivity 0.252ng/mL
Target CSN2
Specificity This assay has high sensitivity and excellent specificity for detection of Casein Beta (CSN2). No significant cross-reactivity or interference between Casein Beta (CSN2) and analogues was observed.
Reactivity Bovine
Sample Types Milk
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately.
Uniprot P02666
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References A modified piggybac transposon system mediated by exogenous mRNA to perform gene delivery in bovine mammary epithelial cells;Up-reCavia (Guinea pig )lation of milk secretion with modified microclimate through manipulating plasminogen-plasmin systemin Murrah buffaloes during hot dry season.;Improved milk production through PG-PL system by provision of in-house shelter management in lactating Murrah buffaloes during winter season.;Proteomic analysis to unravel the effect of heat stress on gene expression and milk synthesis in bovine mammary epithelial cells;Inhibition of arginase via jugular infusion of Nω-hydroxy-nor-l-arginine inhibits casein synthesis in lactating dairy cows;The influence of a modified micro-environment on stress and milk production through the plasminogen-plasmin system in Murrah buffaloes during the hot-humid …;Arginine Supply Impacts the Expression of Candidate microRNA Controlling Milk Casein Yield in Bovine Mammary Tissue;Rationally designed bioactive milk-derived protein scaffolds enhanced new bone formation;
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