| Product Name | CSTB, 1-98aa, Human, Recombinant |
|---|---|
| Description | CSTB, also known as Cystatin B is an anti-protease implicated in myoclonus epilepsy, a degenerative disease of the central nervous system. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. This protein is able to form a dimer stabilized by noncovalent forces and is thought to play a role in protecting against the proteases leaking from lysosomes. In cells, CSTB is located in the lysosomes and the cytoplasm, but also in the nucleus. Recombinant CSTB, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | Cystatin B, CSTB, Stefin B, PME, CST6, CystatinB CHROW21, CPI B, CYTB, EPM1, Liver thiol proteinase inhibitor, STFB. |
| Host | E. coli |
| Molecular Weight | 24.9kDa (219aa), confirmed by MALDI-TOF. |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MMCGAPSATQ PATAETQHIA DQVRSQLEEK ENKKFPVFKA VSFKSQVVAG TNYFIKVHVG DEDFVHLRVF QSLPHENKPL TLSNYQTNKA KHDELTYF |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM TRIS pH 8.0, 20% glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Turk V, et al (1991) FEBS Lett. 285 (2): 213-9. Jarvinen M, et al, (1988) Acta Histochem. 82 (1): 5-18 |
| Background | CSTB, also known as Cystatin B is an anti-protease implicated in myoclonus epilepsy, a degenerative disease of the central nervous system. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. This protein is able to form a dimer stabilized by noncovalent forces and is thought to play a role in protecting against the proteases leaking from lysosomes. In cells, CSTB is located in the lysosomes and the cytoplasm, but also in the nucleus. Recombinant CSTB, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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