| Product Name | CYCS ELISA kit (Human/Mouse/Rat) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Cytochrome C, Somatic (CYCS). No significant cross-reactivity or interference between Cytochrome C, Somatic (CYCS) and analogues was observed. |
| Synonyms | Cyt-C; HCS; CYC; CytC |
| Method | Sandwich |
| Detection Range | 1.56-100ng/mL |
| Sensitivity | 0.55ng/mL |
| Target | CYCS |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Cytochrome C (CYCS). No significant cross-reactivity or interference between Cytochrome C (CYCS) and analogues was observed. |
| Reactivity | Human, Mouse, Rat |
| Sample Types | Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Danhong injection protects cardiomyocytes against hypoxia/reoxygenation- and H2O2-induced injury by inhibiting mitochondrial permeability transition pore opening;Gypenosides alleviate myocardial ischemia-reperfusion injury via attenuation of oxidative stress and preservation of mitochondrial function in rat heart;Plasma Cellular Hypoxia, Mitochondrial Dysfunction, Disease Risk and Prognostic Factors in Type 2 Diabetic Patients in Saudi Arabia.;Taxifolin protects rat against myocardial ischemia/reperfusion injury by modulating the mitochondrial apoptosis pathway;Concentration-Dependent Effects of Zinc Sulfate on DU-145 Human Prostate Cancer Cell Line: Oxidative, Apoptotic, Inflammatory, and Morphological Analyzes; |
| Supplier | Cloud Clone |
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