| Product Name | CYP2E1, 29-493aa, Human (Denatured) |
|---|---|
| Description | CYP2E1 also known as Cytochrome P450 2E1 (abbreviated CYP2E1, EC 1.14.13.n7), is member of the cytochrome P450 mixed-function oxidase system that involved in the metabolism of xenobiotics in the body. While it is involved in the oxidative metabolism of a small range of substrates (mostly small polar molecules), there are many important drug interactions mediated by CYP2E1. Recombinant human CYP2E1, fused to His-tag at N-terminus, was expressed in E.coli. |
| Synonyms | Cytochrome P450 2E1, CPE1, CYP2E, P450-J, P450C2E |
| Host | E. coli |
| Molecular Weight | 38.2kDa (356aa) confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSSWNLPPG PFPLPIIGNL FQLELKNIPK SFTRLAQRFG PVFTLYVGSQ RMVVMHGYKA VKEALLDYKD EFSGRGDLPA FHAHRDRGII FNNGPTWKDI RRFSLTTLRN YGMGKQGNES RIQREAHFLL EALRKTQGQP FDPTFLIGCA PCNVIADILF RKHFDYNDEK FLRLMYLFNE NFHLLSTPWL QLYNNFPSFL HYLPGSHRKV IKNVAEVKEY VSERVKEHHQ SLDPNCPRDL TDCLLVEMEK EKHSAERLYT MDGITVTVAD LFFAGTETTS TTLRYGLLIL MKYPEIEEKL HEEIDRVIGP SRIPAIKDRQ EMPYMDAVVH EIQRFITLVP SNLPHEATRD TIFRGYLIPK GTVVVPTLDS VLYDNQEFPD PEKFKPEHFL NENGKFKYSD YFKPFSTGKR VCAGEGLARM ELFLLLCAIL QHFNLKPLVD PKDIDLSPIH IGFGCIPPRY KLCVIPRS |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in Phosphate buffer saline (pH 7.4) containing 20% glycerol 1mM DTT. |
| Concentration | 0.25mg/ml (determined by Bradford assay) |
| Purity | > 85% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Miller DN., et al. (1965) Ann NY Acad Sci, 119 (3): 957-973. |
| Background | CYP2E1 also known as Cytochrome P450 2E1 (abbreviated CYP2E1, EC 1.14.13.n7), is member of the cytochrome P450 mixed-function oxidase system that involved in the metabolism of xenobiotics in the body. While it is involved in the oxidative metabolism of a small range of substrates (mostly small polar molecules), there are many important drug interactions mediated by CYP2E1. Recombinant human CYP2E1, fused to His-tag at N-terminus, was expressed in E.coli. |
| Supplier | ARP |
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