| Product Name | CYPA ELISA kit (Human) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Cyclophilin A (CYPA). No significant cross-reactivity or interference between Cyclophilin A (CYPA) and analogues was observed. |
| Synonyms | PPIA; CyP-A; CYPH; Peptidylprolyl Isomerase A; Peptidyl-Prolyl Isomerase A; Peptidyl-prolyl cis-trans isomerase A; Cyclosporin A-binding protein |
| Method | Sandwich |
| Detection Range | 1.25-80ng/mL |
| Sensitivity | 0.49ng/mL |
| Target | CYPA |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Cyclophilin A (CYPA). No significant cross-reactivity or interference between Cyclophilin A (CYPA) and analogues was observed. |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Uniprot | P62937 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | EMMPRIN and its ligand cyclophilin A regulate MT1-MMP, MMP-9 and M-CSF during foam cell formation;Cyclophilin-A: a novel biomarker for untreated male essential hypertension;Plasma level of cyclophilin A is increased in patients with type 2 diabetes mellitus and suggests presence of vascular disease;Decrease in Plasma Cyclophilin A Concentration at 1 Month after Myocardial Infarction Predicts Better Left Ventricular Performance and Synchronicity at 6 Months: A Pilot Study in Patients with ST Elevation Myocardial Infarction;Cyclophilin A in Ruptured Intracranial Aneurysm;PPIA rs6850: A> G single-nucleotide polymorphism is associated with raised plasma cyclophilin A levels in patients with coronary artery disease;Urinary Cyclophilin A as a New Marker for Diabetic Nephropathy: A Cross-Sectional Analysis of Diabetes Mellitus;Cyclophilin A enhances macrophage differentiation and lipid uptake in high glucose conditions: a cellular mechanism for accelerated macro vascular disease in diabetes mellitus.;Metformin attenuates effects of cyclophilin A on macrophages, reduces lipid uptake and secretion of cytokines by repressing decreased AMPK activity;Cyclophilin A and CD147 associate with progression of diabetic nephropathy;Association of cyclophilin A level and pulse pressure in predicting recurrence of cerebral infarction;Cyclophilin A/EMMPRIN Axis Is Involved in Pro-Fibrotic Processes Associated with Thoracic Aortic Aneurysm of Marfan Syndrome Patients;APPENDIX A: Relationship between cyclophilin A levels and matrix metalloproteinase-9 activity in cerebrospinal fluid of cognitively normal apolipoprotein E4 carriers …;Urinary podocalyxin and cyclophilin A: markers for early detection of type 2 diabetic nephropathy; |
| Supplier | Cloud Clone |
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