| Product Name | DCUN1D2, 1-259aa, Human |
|---|---|
| Description | DCN1-like protein 2, also known as DCUN1D2, which contains one DCUN1 domain and 1 UBA-like domain, may play a role in the neddylation of cullins that regulate SCF-type ubiquitin ligase complexes. The gene encoding Dcun1D2 exists on human chromosome 13. Chromosome 13 houses key tumor suppressor genes, including BRCA2 and RB1, which are associated with breast cancer susceptibility and retinoblastoma, respectively. Recombinant human DCUN1D2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | defective in cullin neddylation protein 1(DCN1)-like protein 2, DCUN1L2 |
| Host | E. coli |
| Molecular Weight | 58.4 kDa (522aa) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MHKLKSSQKD KVRQFMACTQ AGERTAIYCL TQNEWRLDEA TDSFFQNPDS LHRESMRNAV DKKKLERLYG RYKDPQDENK IGVDGIQQFC DDLSLDPASI SVLVIAWKFR AATQCEFSRK EFLDGMTELG CDSMEKLKAL LPRLEQELKD TAKFKDFYQF TFTFAKNPGQ KGLDLEMAVA YWKLVLSGRF KFLDLWNTFL MEHHKRSIPR DTWNLLLDFG NMIADDMSNY DEEGAWPVLI DDFVEYARPV VTGGKRSLF |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT |
| Concentration | 0.5 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Hassler M., et al. (2007) Mol Cell. 28:371-385. Dunham A., et al. (2004) Nature. 428:522-528. |
| Background | DCN1-like protein 2, also known as DCUN1D2, which contains one DCUN1 domain and 1 UBA-like domain, may play a role in the neddylation of cullins that regulate SCF-type ubiquitin ligase complexes. The gene encoding Dcun1D2 exists on human chromosome 13. Chromosome 13 houses key tumor suppressor genes, including BRCA2 and RB1, which are associated with breast cancer susceptibility and retinoblastoma, respectively. Recombinant human DCUN1D2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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