| Product Name | DHT ELISA kit (General species) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Dihydrotestosterone (DHT). No significant cross-reactivity or interference between Dihydrotestosterone (DHT) and analogues was observed. |
| Synonyms | 5α-DHT; 5α-Dihydrotestosterone; Androstanolone; Stanolone |
| Method | Competitive |
| Detection Range | 30.9-2,500pg/mL |
| Sensitivity | 10.7pg/mL |
| Target | DHT |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Dihydrotestosterone (DHT). No significant cross-reactivity or interference between Dihydrotestosterone (DHT) and analogues was observed. |
| Sample Types | Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix.Incubate 1 hour at 37℃; 3.Aspirate and wash 3 times; 4.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 5.Aspirate and wash 5 times; 6.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 7.Add 50uL Stop Solution.Read at 450 nm immediately. |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Herbicide Metolachlor Causes Changes in Reproductive Endocrinology of Male Wistar Rats;Metabolic Changes and Hormonal Disturbances in Polycystic Ovarian Syndrome Rats and the Amelioration Effects of Metformin and/or Cinnamon Extraction;Treatment of benign prostatic hyperplasia with Croton membranaceus in an experimental animal model;Oral administration of low-dose bisphenol A promotes proliferation of ventral prostate and upregulates prostaglandin D2;Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction by Upregulating PPARα;Dihydrotestosterone Treatment Accelerates Autograft Reversal Sciatic Nerve Regeneration in Rats;Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome;Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression;Syce1 and Syce3 regulate testosterone and dihydrotestosterone synthesis via steroidogenic pathways in mouse Sertoli and Leydig cells; |
| Supplier | Cloud Clone |
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