| Product Name | Disulfide-bond isomerase (DsbC), Recombinant |
|---|---|
| Description | Recombinant Disulfide Oxidoreductase (rDsbA), produced from E.Coli is a periplasmic protein and thioredoxin superfamily member which introduces disulfide bonds directly into substrate proteins by donating the disulfide bond in its active-site Cys30-Pro31-His32-Cys33 to a pair of cysteines in substrate proteins. DsbA consists of 208 amino acids containing signal peptide(1-19 amino acids). Recombinant DsbA (residues 20-208) was expressed in E. coli and purified by using conventional chromatography techniques. |
| Synonyms | dsbC, xprA, Disulfide-bond isomerase (DsbC) E.Coli, Thiol:disulfide interchange protein dsbC, Disulfide-bond isomerase C Thiol:disulfide interchange protein dsbC . |
| Host | E.coli |
| Molecular Weight | 59.4kDa (549aa) |
| Amino Acid Sequence | DDAAIQQTLA KMGIKSSDIQ PAPVAGMKTV LTNSGVLYIT DDGKHIIQGP MYDVSGTAPV NVTNKMLLKQ LNALEKEMIV YKAPQEKHVI TVFTDITCGY CHKLHEQMAD YNALGITVRY LAFPRQGLDS DAEKEMKAIW CAKDKNKAFD DVMAGKSVAP ASCDVDIADH YVLGVQLGVS GTPAVVLSNG TLVPGYQPPK EMKEFLDEHQ KMTSGK |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris pH 8.0, 50mM NaCl, 1mM DTT |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles. |
| References |
Peter W., et al. (2002). EMBO 21, 4774-4784 Laura Segatori., et al. (2004). PNAS 27, 10018-10023 |
| Supplier | ARP |
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