| Product Name | DNMT3L, 1-386aa, Human |
|---|---|
| Description | DNA (cytosine-5)-methyltransferase 3-like isoform, also known as DNMT3L, is a 387 amino acid protein that contains one ADD-type zinc finger and is a member of the DNMT family. Localized to the nucleus and expressed at lows levels in thymus, testis and ovary, DNMT3L does not exhibit DNA methyltransferase activity, but is able to stimulate de novo methylation by DNMT3 and is thought to play a key role in the establishment of genomic imprints. Recombinant human DNMT3L protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | DNA (cytosine-5)-methyltransferase 3-like isoform, DNA (cytosine-5-)-methyltransferase 3-like |
| Host | E. coli |
| Molecular Weight | 32.9 kDa(302aa) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSEFMAAIP ALDPEAEPSM DVILVGSSEL SSSVSPGTGR DLIAYEVKAN QRNIEDICIC CGSLQVHTQH PLFEGGICAP CKDKFLDALF LYDDDGYQSY CSICCSGETL LICGNPDCTR CYCFECVDSL VGPGTSGKVH AMSNWVCYLC LPSSRSGLLQ RRRKWRSQLK AFYDRESENP LEMFETVPVW RRQPVRVLSL FEDIKKELTS LGFLESGSDP GQLKHVVDVT DTVRKDVEEW GPFDLVYGAT PPLGHTCDRP PSWYLFQFHR LLQYARPKPG SPRPFFWMFV DNLVLNKEDL DVASRFLEME PVTIPDVHGG SLQNAVRVWS NIPAIRSSRH WALVSEEELS LLAQNKQSSK LAAKWPTKLV KNCFLPLREY FKYFSTELTS SL |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.4M Urea |
| Concentration | 1mg/ml (determined by Bradford assay) |
| Purity | > 80% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Yoder J A., et al. (1997) J Mol Biol. 270: 385-395. Suetake I., et al. (2006) J Biochem 1405: 553-559. |
| Background | DNA (cytosine-5)-methyltransferase 3-like isoform, also known as DNMT3L, is a 387 amino acid protein that contains one ADD-type zinc finger and is a member of the DNMT family. Localized to the nucleus and expressed at lows levels in thymus, testis and ovary, DNMT3L does not exhibit DNA methyltransferase activity, but is able to stimulate de novo methylation by DNMT3 and is thought to play a key role in the establishment of genomic imprints. Recombinant human DNMT3L protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
All Research Products are sold for laboratory RESEARCH USE ONLY and ARE NOT TO BE USED FOR HUMAN OR ANIMAL THERAPEUTIC OR DIAGNOSTIC APPLICATIONS. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. ARP American Research Products, Inc. does not submit its products for regulatory review by any government body or other organization, and we do not validate them for clinical, therapeutic or diagnostic use, or for safety and effectiveness. You are solely responsible for making sure that the way you use the products complies with applicable laws, regulations and governmental policies and for obtaining all necessary approvals, intellectual property rights, licenses and permissions that you may need related to your use. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process. ARP American Research Products, Inc. disclaims any and all representations or warranties of any kind whatsoever, express or implied, including without limitation any implied warranties of merchantability or fitness for a particular purpose, of non-infringement, or regarding results obtained through the use of any product, whether arising from a statute or otherwise in law or from a course of performance, dealing or usage of trade.
