| Product Name | Canine Aatestatin (CAT) ELISA Kit |
|---|---|
| Description | Canine Aatestatin (CAT) ELISA Kit has high sensitivity and excellent specificity for detection of Canine CAT. No significant cross-reactivity or interference between Canine CAT and analogues was observed. Catalase is a common enzyme found in nearly all living organisms which are exposed to oxygen, where it functions to catalyze the decomposition of hydrogen peroxide to water and oxygen.Catalase has one of the highest turnover numbers of all enzymes; one molecule of catalase can convert millions of molecules of hydrogen peroxide to water and oxygen per second.Catalase is a tetramer of four polypeptide chains, each over 500 amino acids long. It contains four porphyrin heme (iron) groups that allow the enzyme to react with the hydrogen peroxide. The optimum pH for human catalase is approximately 7,and has a fairly broad maximum (the rate of reaction does not change appreciably at pHs between 6.8 and 7.5). This Canine Aatestatin (CAT) ELISA Kit employs a two-site sandwich ELISA to quantitate CAT in samples. An antibody specific for CAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CAT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CAT bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | CAT |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Canine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Storage | 2-8°C |
| Supplier | Abbkine |
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