Canine Adrencocorticotropic Hormone (ACTH) ELISA Kit

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KTE20033
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Product Name Canine Adrencocorticotropic Hormone (ACTH) ELISA Kit
Description Canine Adrencocorticotropic Hormone (ACTH) ELISA Kit has high sensitivity and excellent specificity for detection of Canine ACTH. No significant cross-reactivity or interference between Canine ACTH and analogues was observed. ACTH (Adrenocorticotropic hormone) or corticotropin is a 39-amino acid peptide hormone (MW=4500) secreted by the pituitary to regulate the production of steroid hormones by the adrenal cortex. ACTH secretion from the anterior pituitary is controlled by both a classical negative feedback control mechanism and CNS-stress mediated control system. Various types of stress or pain perceived in higher levels of the brain modulate secretion of the hypothalamic neurosecretory hormone, corticotropin releasing hormone (CRH), a 41-amino acid peptide. CRH stimulates pituitary ACTH secretion. The second peptide that modulates ACTH secretion is vasopressin (AVP). AVP secretion is also stimulated by stress and acts synergistically with CRH to increase ACTH secretion in the pituitary portal circulation. ACTH increases the synthesis and release of all adrenal sterioids, aldosterone, cortisol and adrenal androgens. This Canine Adrencocorticotropic Hormone (ACTH) ELISA Kit employs a two-site sandwich ELISA to quantitate ACTH in samples. An antibody specific for ACTH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACTH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACTH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACTH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Synonyms ACTH
Method Sandwich ELISA
Detection Range Please inquire
Sensitivity Please inquire
Reactivity Canine
Sample Types Cell culture supernatants, Serum, Plasma, Other biological fluids
Storage 2-8°C
Supplier Abbkine

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