| Product Name | Canine Angiotension 1-7 (Ang1-7) ELISA Kit |
|---|---|
| Description | Canine Angiotension 1-7 (Ang1-7) ELISA Kit has high sensitivity and excellent specificity for detection of Canine Ang1-7. No significant cross-reactivity or interference between Canine Ang1-7 and analogues was observed. Angiotensin(1-7) (Ang1-7) acting at the level of the rostral ventrolateral medulla (RVLM) affects arterial pressure. Astroglia are a plausible cellular target of Ang1-7 in RVLM. Astrocytic responsiveness to Ang1-7 is reduced in SHR. Ang1-7 modulates astrocytic signalling which in vivo may affect local metabolism and microcirculation, resulting in changes in activity of RVLM pre-sympathetic neurones and hence blood pressure.Ang1-7 is an important component of the central renin-angiotensin system and affects haemodynamics when injected into the RVLM. Effect of Ang1-7 could be blocked by co-application of its antagonist, A779 (200 nM) but not losartan (1 µM). non-CA neurones and glia in RVLM are the primary targets for Ang1-7 and its effect on [Ca2+]i in non-CA neurones is enhanced in SHR. This Canine Angiotension 1-7 (Ang1-7) ELISA Kit employs a two-site sandwich ELISA to quantitate Ang1-7 in samples. An antibody specific for Ang1-7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAng1-7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Ang1-7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Ang1-7 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | Ang1-7 |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Canine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Storage | 2-8°C |
| Supplier | Abbkine |
All Research Products are sold for laboratory RESEARCH USE ONLY and ARE NOT TO BE USED FOR HUMAN OR ANIMAL THERAPEUTIC OR DIAGNOSTIC APPLICATIONS. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. ARP American Research Products, Inc. does not submit its products for regulatory review by any government body or other organization, and we do not validate them for clinical, therapeutic or diagnostic use, or for safety and effectiveness. You are solely responsible for making sure that the way you use the products complies with applicable laws, regulations and governmental policies and for obtaining all necessary approvals, intellectual property rights, licenses and permissions that you may need related to your use. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process. ARP American Research Products, Inc. disclaims any and all representations or warranties of any kind whatsoever, express or implied, including without limitation any implied warranties of merchantability or fitness for a particular purpose, of non-infringement, or regarding results obtained through the use of any product, whether arising from a statute or otherwise in law or from a course of performance, dealing or usage of trade.
