| Product Name | dsSafe Gel Staining Solution |
|---|---|
| Description | The dsSafe dye is a full structural analog of SYBR™ Safe and a fluorescent dye for nucleic acid staining in agarose and polyacrylamide gels. dsSafe is the best and safer alternative to ethidium bromide (EtBr); it was shown to be less toxic, non-mutagenic, and non-carcinogenic. dsSafe allows nucleic acid visualization with sensitivity similar to that of ethidium bromide. Dye excitation can be performed with both conventional UV transilluminators and blue light transilluminators, with the latter ones being safer for the eyes and nucleic acid sample. When blue light is used for dsSafe excitation, background fluorescence is lower than in the case of ethidium bromide excitation with UV light. Excised DNA fragments for subsequent cloning are not exposed to damaging UV radiation, thus increasing cloning efficacy and reducing the frequency of adverse mutations caused by UV. The spectrum of the DNA-dye complex has two excitation maxima [in the UV (280 nm) and blue ranges (502 nm)] and one emission maximum in the green range (~530 nm). Available as 10,000X concentrate in DMSO. To stain the gel, add the dye to melted agarose solution or stain the gel after gel electrophoresis in TAE/TBE buffer for 10-20 minutes. This dye is non-mutagenic, non-carcinogenic, and far less toxic for DNA and RNA staining. Its high sensitivity is identical to that of EtBr. Excitation is possible with both UV and blue light. Easy to use dye; the dye can be added to melted agarose solution or the gel can be stained within 10 to 20 minutes after gel electrophoresis completion. Can be used for all routine laboratory procedures requiring nucleic acid visualization. Does not require special storage or disposal conditions. For nucleic acid gel staining, we also offer dsGreen Gel Staining Solution with higher sensitivity to DNA . However, in contrast with dsSafe, gel staining with dsGreen Gel Staining Solution is recommended only after the completion of gel electrophoresis. |
| Form | Orange solution. |
| Concentration | 10000X |
| Storage | Shipped at room temperature. Upon delivery, store in the dark at -20°C. Avoid prolonged exposure to light. Desiccate. |
| Supplier | Antibodies.com |
All Research Products are sold for laboratory RESEARCH USE ONLY and ARE NOT TO BE USED FOR HUMAN OR ANIMAL THERAPEUTIC OR DIAGNOSTIC APPLICATIONS. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. ARP American Research Products, Inc. does not submit its products for regulatory review by any government body or other organization, and we do not validate them for clinical, therapeutic or diagnostic use, or for safety and effectiveness. You are solely responsible for making sure that the way you use the products complies with applicable laws, regulations and governmental policies and for obtaining all necessary approvals, intellectual property rights, licenses and permissions that you may need related to your use. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process. ARP American Research Products, Inc. disclaims any and all representations or warranties of any kind whatsoever, express or implied, including without limitation any implied warranties of merchantability or fitness for a particular purpose, of non-infringement, or regarding results obtained through the use of any product, whether arising from a statute or otherwise in law or from a course of performance, dealing or usage of trade.
