| Product Name | DUSP21, 1-190aa, Human |
|---|---|
| Description | DUSP21 (Dual specificity protein phosphatase 21) belongs to the protein-tyrosine phosphatase family. Dual specificity protein phosphatases(DUSPs) inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. This enzyme can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine. It is exclusively expressed in testis where is preferentially dephosphorylates phosphotyrosine residues in MAPK peptides. Recombinant human DUSP21 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | Dual specificity protein phosphatase 21, |
| Host | E. coli |
| Molecular Weight | 35.5 kDa (314aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSHMTASAS SFSSSQGVQQ PSIYSFSQIT RSLFLSNGVA ANDKLLLSSN RITAIVNASV EVVNVFFEGI QYIKVPVTDA RDSRLYDFFD PIADLIHTID MRQGRTLLHC MAGVSRSASL CLAYLMKYHS MSLLDAHTWT KSRRPIIRPN NGFWEQLINY EFKLFNNNTV RMINSPVGNI PDIYEKDLRM MISM |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Hood, K.L., et al. (2002) Biochem. Biophys. Res. Commun. 298: 545-551. Keyse, S.M. (1995) Biochim. Biophys. Acta 1265: 152-160. |
| Background | DUSP21 (Dual specificity protein phosphatase 21) belongs to the protein-tyrosine phosphatase family. Dual specificity protein phosphatases(DUSPs) inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. This enzyme can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine. It is exclusively expressed in testis where is preferentially dephosphorylates phosphotyrosine residues in MAPK peptides. Recombinant human DUSP21 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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