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|Product Name||Ecotin, 21-162aa|
|Amino Acid Sequence||MGSSHHHHHH SSGLVPRGSH MAESVQPLEK IAPYPQAEKG MKRQVIQLTP QEDESTLKVE LLIGQTLEVD CNLHRLGGKL ENKTLEGWGY DYYVFDKVSS PVSTMMACPD GKKEKKFVTA YLGDAGMLRY NSKLPIVVYT PDNVDVKYRV WKAEEKIDNA VVR|
|Background||Ecotin (E. coli serine protease inhibitor) inhibits pancreatic serine proteases. This protein inhibits chymotrypsin, trypsin, elastases, factor X, kallikrein as well as a variety of other proteases. The strength of inhibition does not appear to be correlated with a particular protease specificity. Recombinant E. coli Ecotin protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Concentration||0.5 mg/ml (determined by Bradford assay)|
|Form||Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 100mM NaCl|
|Intended Use||For Research Use Only|
|Molecular Weight||35.1 kDa (304aa) confirmed by MALDI-TOF (molecular weight on SDS-PAGE will appear higher)|
|NCBI Accession #||NP_416713|
|Purity||> 95% by SDS-PAGE|
|References||Lilla JN., et al. (2009) J Biol Chem. 284(20):13792-803.
Lund LR., et al. (2006) EMBO J. 25(12):2686-97.
|Storage||Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.|
|Synonyms||ECK2201, eti, JW2197|
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