| Product Name | EDN1 ELISA kit (Rat) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Endothelin 1 (EDN1). No significant cross-reactivity or interference between Endothelin 1 (EDN1) and analogues was observed. |
| Synonyms | ET1; PPET1; Preproendothelin-1; Big endothelin-1 |
| Method | Competitive |
| Detection Range | 6.17-500pg/mL |
| Sensitivity | 2.71pg/mL |
| Target | EDN1 |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Endothelin 1 (EDN1). No significant cross-reactivity or interference between Endothelin 1 (EDN1) and analogues was observed. |
| Reactivity | Rat |
| Sample Types | Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix.Incubate 1 hour at 37℃; 3.Aspirate and wash 3 times; 4.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 5.Aspirate and wash 5 times; 6.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 7.Add 50uL Stop Solution.Read at 450 nm immediately. |
| Uniprot | P22388 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Attenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat model;Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation;Pivotal role of mast cell carboxypeptidase A in mediating protection against small intestinal ischemia–reperfusion injury in rats after ischemic preconditioning;Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation;Abnormal Retinal Structure and Function of Mother Wistar Rats Supplemented Aspartame, Glutamate and Galactose;Antioxidant and anti-inflammatory activities of a commercial noni juice revealed by carrageenan-induced paw edema;A Study of Effect of Experimental Pre-Eclampsia on Plasma Lipocalin-2 Level in Rats.;Antihypertensive effects of Ocimum gratissimumextract: Angiotensin-converting enzyme inhibitor in vitro and in vivo investigation;Improved endogenous epoxyeicosatrienoic acid production mends heart function via increased PGC 1α-mitochondrial functions in metabolic syndrome;Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in rats;Tianma Gouteng Decoction Exerts Cardiovascular Protection by Upregulating OPG and TRAIL in Spontaneously Hypertensive Rats;Effect of liraglutide on microcirculation in rat model with absolute insulin deficiency;Post-stroke treatment of storax improves long-term outcomes of stroke in rats;Laser Doppler flowmetry in assessing the effect of liraglutide on microcirculation in rats with absolute insulin deficiency; |
| Supplier | Cloud Clone |
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