| Product Name | EEF2, 574-858aa, Human (Denatured) |
|---|---|
| Description | EEF2 is a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. Recombinant human EEF2 protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Synonyms | elongation factor 2, EEF-2, EF-2, EF2 |
| Host | E. coli |
| Molecular Weight | 14.7kDa(131aa) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSDPVVSYR ETVSEESNVL CLSKSPNKHN RLYMKARPFP DGLAEDIDKG EVSARQELKQ RARYLAEKYE WDVAEARKIW CFGPDGTGPN ILTDITKGVQ YLNEIKDSVV AGFQWATKEG ALCEENMRGV RFDVHDVTLH ADAIHRGGGQ IIPTARRCLY ASVLTAQPRL MEPIYLVEIQ CPEQVVGGIY GVLNRKRGHV FEESQVAGTP MFVVKAYLPV NESFGFTADL RSNTGGQAFP QCVFDHWQIL PGDPFDNSSR PSQVVAETRK RKGLKEGIPA LDNFLDKL |
| Tag | His-tag |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 50% glycerol, 2mM DTT, 2mM EDTA |
| Concentration | 0.25 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Yamashita A., et al (2009). Genes Dev. 23:1091-1105 Olsen J.V., et al (2006). Cell 127:635-648 |
| Background | EEF2 is a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. Recombinant human EEF2 protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Supplier | ARP |
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