| Product Name | EEF2K, 1-725aa, Human |
|---|---|
| Description | Eukaryotic elongation factor-2 kinase, also known as EEF2K, is threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. Recombinant human EEF2K protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | Eukaryotic elongation factor-2 kinase, eEF-2K, HSu93850, eEF2K |
| Host | E. coli |
| Molecular Weight | 23.1kDa (213aa) confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMADEDLI FRLEGVDGGQ SPRAGHDGDS DGDSDDEEGY FICPITDDPS SNQNVNSKVN KYYSNLTKSE RYSSSGSPAN SFHFKEAWKH AIQKAKHMPD PWAEFHLEDI ATERATRHRY NAVTGEWLDD EVLIKMASQP FGRGAMRECF RTKKLSNFLH AQQWKGASNY VAKRYIEPVD RDVYFEDVRL QMEAKLWGEE YNRHKPPKQV DIMQMCIIEL KDRPGKPLFH LEHYIEGKYI KYNSNSGFVR DDNIRLTPQA FSHFTFERSG HQLIVVDIQG VGDLYTDPQI HTETGTDFGD GNLGVRGMAL FFYSHACNRI CESMGLAPFD LSPRERDAVN QNTKLLQSAK TILRGTEEKC GSPRVRTLSG SRPPLLRPLS ENSGDENMSD VTFDSLPSSP SSATPHSQKL DHLHWPVFSD LDNMASRDHD HLDNHRESEN SGDSGYPSEK RGELDDPEPR EHGHSYSNRK YESDEDSLGS SGRVCVEKWN LLNSSRLHLP RASAVALEVQ RLNALDLEKK IGKSILGKVH LAMVRYHEGG RFCEKGEEWD QESAVFHLEH AANLGELEAI VGLGLMYSQL PHHILADVSL KETEENKTKG FDYLLKAAEA GDRQSMILVA RAFDSGQNLS PDRCQDWLEA LHWYNTALEM TDCDEGGEYD GMQDEPRYMM LAREAEMLFT GGYGLEKDPQ RSGDLYTQAA EAAMEAMKGR LANQYYQKAE EAWAQMEE |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in Phosphate Buffered Saline (pH7.4) containing 10% glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 85% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Ryazanov A.G., et al. (1997) Proc. Natl. Acad. Sci. u.S.A. 94:4884-4889 Knebel A., et al. (2001) EMBO J. 20:4360-4369 |
| Background | Eukaryotic elongation factor-2 kinase, also known as EEF2K, is threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. Recombinant human EEF2K protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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