| Product Name | EGF ELISA kit (Rat) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Epidermal Growth Factor (EGF). No significant cross-reactivity or interference between Epidermal Growth Factor (EGF) and analogues was observed. |
| Synonyms | URG; Beta-Urogastrone; Pro-epidermal growth factor |
| Method | Sandwich |
| Detection Range | 15.6-1,000pg/mL |
| Sensitivity | 6.0pg/mL |
| Target | EGF |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Epidermal Growth Factor (EGF). No significant cross-reactivity or interference between Epidermal Growth Factor (EGF) and analogues was observed. |
| Reactivity | Rat |
| Sample Types | Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Uniprot | P07522 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass model;The Probiotic Mixture VSL#3 Accelerates Gastric Ulcer Healing by Stimulating Vascular Endothelial Growth Factor;Allogeneic transplantation of an adipose-derived stem cell (ASC) sheet combined with artificial skin accelerates wound healing in a rat wound model of type 2 diabetes and obesity;Polydopamine nanoparticles modulating stimuli-responsive PNIPAM hydrogels with cell/tissue adhesiveness.;The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy rats;Human adipose-derived stem cell-loaded small intestinal submucosa as a bioactive wound dressing for the treatment of diabetic wounds in rats;Beetroot Improves the Therapeutic Efficacy of Bone Marrow-Derived Mesenchymal Stem Cells in Preventing Cisplatin Induced Nephrotoxicity in Male Rats; |
| Supplier | Cloud Clone |
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