EliKine™ Mouse GM-CSF ELISA Kit

Category: Assay Kits
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KET7002
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Product Name EliKine™ Mouse GM-CSF ELISA Kit
Description EliKine™ Mouse GM-CSF ELISA Kit has high sensitivity and excellent specificity for detection of Mouse GM-CSF. No significant cross-reactivity or interference between Mouse GM-CSF and analogues was observed. Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim. Unlike granulocyte colony-stimulating factor, which specifically promotes neutrophil proliferation and maturation, GM-CSF affects more cell types, especially macrophages and eosinophils. EliKine™ Mouse GM-CSF ELISA Kit employs a two-site sandwich ELISA to quantitate GM-CSF in samples. An antibody specific for GM-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GM-CSF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GM-CSF is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GM-CSF bound in the initial step. The color development is stopped and the intensity of the color is measured.
Synonyms GM-CSF, GMCSF, Colony stimulating factor, Granulocyte Macrophage Colony Stimulating Factor, CSF 2, CSF2
Method Sandwich ELISA
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 4 pg/mL
Reactivity Mouse
Sample Types Cell culture supernatants, Serum, Plasma, Other biological fluids
Gene Id 12981
Storage 2-8°C
Supplier Abbkine

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