EliKine™ Mouse IFN-γ ELISA Kit

Category: Assay Kits
Catalog
KET7003
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Product Name EliKine™ Mouse IFN-γ ELISA Kit
Description EliKine™ Mouse IFN-γ ELISA Kit has high sensitivity and excellent specificity for detection of Mouse IFN-γ. No significant cross-reactivity or interference between Mouse IFN-γ and analogues was observed. IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits. EliKine™ Mouse IFN-γ ELISA Kit employs a two-site sandwich ELISA to quantitate IFN-γ in samples. An antibody specific for IFN-γ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-γ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFN-γ is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFN-γ bound in the initial step. The color development is stopped and the intensity of the color is measured.
Synonyms IFG, IFI, IFN gamma, IFN Immune, IFNG, Interferon gamma, Interferon Gamma Precursor, Macrophage Activating Factor, MAF, T Cell Interferon, Type II Interferon
Method Sandwich ELISA
Detection Range 31.25 pg/ml- 2000 pg/ml
Sensitivity 16 pg/ml
Reactivity Mouse
Sample Types Cell culture supernatants, Serum, Plasma, Other biological fluids
Gene Id 15978
Storage 2-8°C
Supplier Abbkine

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