EliKine™ Mouse IL-5 ELISA Kit

Category: Assay Kits
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KET7008
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Product Name EliKine™ Mouse IL-5 ELISA Kit
Description EliKine™ Mouse IL-5 ELISA Kit has high sensitivity and excellent specificity for detection of Mouse IL-5. No significant cross-reactivity or interference between Mouse IL-5 and analogues was observed. IL-5 (Interleukin-5) is a secreted cytokine that is expressed as a covalent dimer. It is produced by activated Th2 cells, eosinophils, mast cells, and iNKT cells and promotes eosinophilic and basophilic differentiation and activation. IL-5 signals through a receptor complex containing IL-5 R alpha and the Common beta Chain/CD131 on eosinophils and basophils. Naturally occurring soluble forms of IL-5 R alpha retain the ability to bind IL-5 and limit its bioavailability. EliKine™ Mouse IL-5 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-5 in samples. An antibody specific for IL-5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-5 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Synonyms IL 5, IL5, IL-5, IL-5T-cell replacing factor, interleukin-5, TRF, TRFB cell differentiation factor I
Method Sandwich ELISA
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 4 pg/mL
Reactivity Mouse
Sample Types Cell culture supernatants, Serum, Plasma, Other biological fluids
Gene Id 16191
Storage 2-8°C
Supplier Abbkine

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