| Product Name | EliKine™ Mouse VEGF ELISA Kit |
|---|---|
| Description | EliKine™ Mouse VEGF ELISA Kit has high sensitivity and excellent specificity for detection of Mouse VEGF-A. No significant cross-reactivity or interference between Mouse VEGF-A and analogues was observed. VEGF (Vascular endothelial growth factor) is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is required for regulating the proliferation, migration, and survival of embryonic endothelial cells and during wound healing and the female reproductive cycle in adults. Pathologically, it is involved in tumor development and tumor vascular leakage. VEGF binds to VEGF R1/Flt-1, VEGF R2/Flk-1/KDR, and Neuropilin-1. Multiple forms of VEGF are generated by alternative splicing and proteolysis. EliKine™ Mouse VEGF ELISA Kit employs a two-site sandwich ELISA to quantitate VEGF-A in samples. An antibody specific for VEGF-A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VEGF-A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VEGF-A is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VEGF-A bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | VEGFA, RP1-261G23.1, MGC70609, MVCD1, VEGF, VEGF-A, VPF, vascular endothelial growth factor isoform VEGF165, vascular permeability factor |
| Method | Sandwich ELISA |
| Detection Range | 15.6 pg/ml-1000 pg/ml |
| Sensitivity | 8 pg/ml |
| Reactivity | Mouse |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 22339 |
| Storage | 2-8°C |
| Supplier | Abbkine |
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