| Product Name | EliKine™ Rat Interferon Gamma (IFN-γ) ELISA Kit |
|---|---|
| Description | EliKine™ Rat IFN-γ ELISA Kit has high sensitivity and excellent specificity for detection of Rat IFN-γ. No significant cross-reactivity or interference between Rat IFN-γ and analogues was observed. IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits. EliKine™ Rat Interferon Gamma (IFN-γ) ELISA Kit employs a two-site sandwich ELISA to quantitate IFN-γ in samples. An antibody specific for IFN-γ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-γ present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Rat IFN-γ detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of IFN-γ bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | IFG, IFI, IFN gamma, IFN Immune, IFNG, Interferon gamma, Interferon Gamma Precursor, Macrophage Activating Factor, MAF, T Cell Interferon, Type II Interferon |
| Method | Sandwich ELISA |
| Detection Range | 150 pg/mL-2400 pg/mL |
| Sensitivity | 15 pg/mL |
| Reactivity | Rat |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 25712 |
| Storage | 2-8°C |
| Supplier | Abbkine |
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