EliKine™ Rat IL-17 ELISA Kit

Category: Assay Kits
Catalog
KET9005
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Product Name EliKine™ Rat IL-17 ELISA Kit
Description EliKine™ Rat IL-17 ELISA Kit has high sensitivity and excellent specificity for detection of Rat IL-17. No significant cross-reactivity or interference between Rat IL-17 and analogues was observed. IL-17 is a pro-inflammatory cytokine that is secreted by a subset of activated T cells. It is a disulfide-linked homodimer with both glycosylated and non-glycosylated forms. IL-17A induces stromal cells to produce pro-inflammatory and hematopoietic cytokines, and also enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts. EliKine™ Rat IL-17 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-17 in samples. An antibody specific for IL-17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-17 is added to the wells. After washing, proprietary EliKine™ Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-17 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Synonyms IL 17, IL17A, CTLA8, IL-17, IL-17A, IL17, cytotoxic T-lymphocyte-associated antigen 8, cytotoxic T-lymphocyte-associated protein 8, cytotoxic T-lymphocyte-associated serine esterase 8, interleukin 17 (cytotoxic T-ly
Method Sandwich ELISA
Detection Range 3.13 pg/mL-200 pg/mL
Sensitivity 2 pg/mL
Reactivity Rat
Sample Types Cell culture supernatants, Serum, Plasma, Other biological fluids
Gene Id 301289
Storage 2-8°C
Supplier Abbkine

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